Studies on Glycoproteins
نویسندگان
چکیده
منابع مشابه
Further Studies on the Interaction Between Human Platelet Membrane Glycoproteins
labeled membrane proteins by density gradient ultracentrifugation using 1 O%-40% sucrose gradients containing the nonionic detergent. Studies were performed using soluble proteins derived from membranes isolated in the presence or absence of EDTA. Analysis of gradient fractions by SDS-polyacrylamide gel electrophoresis showed that in the absence of divalent cation chelation. GP lIb and lIla pen...
متن کاملStudies on the chemical and enzymatic modification of glycoproteins. A general method for the tritiation of sialic acid-containing glycoproteins.
A general method is described for the radioactive labeling of proteins containing terminal sialic acid in the carbohydrate moieties. Quantitative conversion of these residues to a radioactive 7-carbon analogue of sialic acid was achieved by sequential periodate oxidation and tritiated borohydride reduction. Evidence is presented to establish the identity of the radioactive derivative as 5-aceta...
متن کاملPaper electrophoretic studies of glycoproteins in rheumatic fever.
TmCLASSIC REPORT by Longsworth et at. (1) in 1939 on serum protein fractionation by free electrophoresis included rheumatic fever as one of the febrile diseases that had been studied. Employing the same medium, Rutstein et at. (2) followed this work with a more detailed study of serum proteins in rheumatic fever and noted changes in total protein and gamma globulin concentrations. Shetlar and c...
متن کاملCell surface glycoproteins: biochemical, immunological and molecular biological studies.
This article briefly summarizes the author's contribution to the study of cell surface glycoproteins. At first, much effort was devoted to developing analytical methods, especially exoglycosidases and endoglycosidases. An endo-beta-N-acetylglucosaminidase found in Streptococcus pneumoniae was the first example of an endoglycosidase acting on glycoproteins. A combination of radioactive labeling,...
متن کاملStudies of membrane glycoproteins in the rough endoplasmic reticulum.
Studies on membrane glycoproteins which have the RER as their final destination are comparatively rare; thus it has not been possible to examine the initial stages of protein compartmentalization. The roles of the RER and the Golgi apparatus in membrane protein sorting need to be elucidated. It has been suggested, for example, that RER proteins would be returned to the endoplasmic reticulum whi...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1970
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)62727-9